New techniques to assess whole-sediment toxicity for two Australian freshwater species

By Tyler Melher, Vincent Pettigrove and Michael Keough

New whole-sediment TIE techniques with two Australian freshwater macroinvertebrates

Development of whole-sediment toxicity identification evaluation (TIE) techniques for two Australian freshwater species: Chironomus tepperi and Austrochiltonia subtenuis.

This article was published online in Environmental Toxicology and Chemistry on 7th March 2017 and can be found here  http://doi: 10.1002/etc.3787. For more information please contact Vincent Pettigrove


Most of the public literature and available guidance documents on the conduct of freshwater whole-sediment toxicity identification evaluations (TIEs) detail the use of test organisms and amending agents that are readily available in North America. These commonly used test organisms and the supported amending agents, however, are not available and largely inappropriate (i.e. not native species) for conducting whole-sediment TIEs outside of North America. The overall objective of the present study was to build foundational methods for performing freshwater whole-sediment TIEs in Australia. We examined the capability of three amending agents: ANZ38 Zeolite (for ammonia), Oxpure 325B-9 Activated Carbon (for non-polar organics), and Lewatit MonoPlus TP 207 (for cationic metals) on two Australian native freshwater species: the midge Chironomus tepperi and the amphipod Austrochiltonia subtenuis. To evaluate the effectiveness of each amendment, bioassays were conducted with spiked sediments of ammonia, permethrin (as part of a commerical formulation), and copper using acute median lethal concentrations (LC50s) for both species and growth (EC50) of midges as the endpoints of interest. This article is protected by copyright. All rights reserved.

KEYWORDS: Australia; Freshwater toxicology; Sediment toxicity; Toxic identification evaluations (TIEs).